see also: Twitter (@renkawitzteam) & Youtube

Lifeact-GFP (actin cytoskeleton marker) expressing dendritic cell migrating towards a chemokine source (CCL19) along 'pore size decision microchannels. GM-CSF-differentiated and LPS-stimulated dendritic cells (DCs); PDMS-based microchannels attached to a non-coated glass surface; imaged with a Leica DMi8 microscope. (see also Kroll et al, Current Protocols 2022)

NATURE VIDEOS highlighting the findings of Jörg's postdoctoral research at IST Austria.

Dendritic cell migration towards a chemokine source (CCL19) along 'highway-like' microchannels. Movie by Madeleine. (GM-CSF-differentiated and LPS-stimulated dendritic cells (DCs); PDMS-based microchannels attached to a non-coated glass surface; imaged with a Leica DMi8 microscope). 

Dendritic cell migration in microenvironmental confinement. Mouse dendritic cell migrating in microenvironmental confinement between a layer of agarose and a glass cover slide (UnderAgarose Assay). The actin cytoskeleton is labeled by Lifeact-GFP and shown in black on the right. GM-CSF-differentiated and LPS-stimulated mouse dendritic cell (DC). Imaged with a Leica DMi8 microscope - for further details see 'Clausen et al, Guidelines for mouse and human DC functional assays, European Journal of Immunology 2022', chapter 6.

Dendritic cell migration in a 3D collagen matrix towards a chemokine (CCL19) source. (GM-CSF-differentiated and LPS-stimulated dendritic cell (DC); bovine collagen; the cellular nucleus is stained with Hoechst and shown in blue; imaged with a Nikon Ecplise Ti).

Macrophage motility in a pillar forest mimicking complex microenvironments. (M-CSF differentiated mouse bone-marrow-derived macrophage; PDMS-based micropillars; imaged with a Nikon Ecplise Ti).

The nucleus (cyan) as a mechanical gauge to probe pore sizes and thus enable migration along the path of least resistance. (GM-CSF-differentiated and LPS-stimulated dendritic cell (DC); PDMS-based microchannels attached to a non-coated glass surface;  the cellular nucleus is stained with Hoechst and shown in cyan; imaged with a Nikon Ecplise Ti.).