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CETN2-GFP expressing dendritic cell navigating along a path decision and breaking its centrosome. GM-CSF-differentiated and LPS-stimulated dendritic cells (DCs); Dyrk3 inhibition causes centrosome breakage as indicated by the separation of the pair of centrioles (shown in black; see also the red arrows); the nucleus is shown in blue; PDMS-based microchannels attached to a non-coated glass surface; imaged with a Leica DMi8 microscope.

(for further details, see Schmitt et al, Science Advances 2025)

Myosin-GFP expressing dendritic cell navigating in a microenvironmental maze and repositioning its nucleus. GM-CSF-differentiated and LPS-stimulated dendritic cells (DCs); PDMS-based pillar forrests attached to a non-coated glass surface; imaged with a Leica DMi8 microscope.

(for further details, see Kroll et al, EMBO J 2023)

Dendritic cell navigating through a path junction with competing mechanical and chemical guidance cues. Nucleus is shown in cyan. GM-CSF-differentiated and LPS-stimulated dendritic cells (DCs); PDMS-based pillar forrests attached to a non-coated glass surface; imaged with a Leica DMi8 microscope. (for further details, see Kroll et al, EMBO J 2023)

Lifeact-GFP (actin cytoskeleton marker) expressing dendritic cell migrating towards a chemokine source (CCL19) along 'pore size decision microchannels. GM-CSF-differentiated and LPS-stimulated dendritic cells (DCs); PDMS-based microchannels attached to a non-coated glass surface; imaged with a Leica DMi8 microscope.

(for further details, see Kroll et al, Current Protocols 2022)

NATURE VIDEOS highlighting the findings of Jörg's postdoctoral research at IST Austria.

(for further details, see Renkawitz et al, Nature 2019)

Dendritic cell migration towards a chemokine source (CCL19) along 'highway-like' microchannels. Movie by Madeleine. (GM-CSF-differentiated and LPS-stimulated dendritic cells (DCs); PDMS-based microchannels attached to a non-coated glass surface; imaged with a Leica DMi8 microscope). 

Dendritic cell migration in microenvironmental confinement. Mouse dendritic cell migrating in microenvironmental confinement between a layer of agarose and a glass cover slide (UnderAgarose Assay). The actin cytoskeleton is labeled by Lifeact-GFP and shown in black on the right. GM-CSF-differentiated and LPS-stimulated mouse dendritic cell (DC). Imaged with a Leica DMi8 microscope.

(for further details see 'Clausen et al, Guidelines for mouse and human DC functional assays, European Journal of Immunology 2022', chapter 6.) 

Dendritic cell migration in a 3D collagen matrix towards a chemokine (CCL19) source. (GM-CSF-differentiated and LPS-stimulated dendritic cell (DC); bovine collagen; the cellular nucleus is stained with Hoechst and shown in blue; imaged with a Nikon Ecplise Ti).

Macrophage motility in a pillar forest mimicking complex microenvironments. (M-CSF differentiated mouse bone-marrow-derived macrophage; PDMS-based micropillars; imaged with a Nikon Ecplise Ti).

(for further details, see Renkawitz et al, Methods in Cell Biology 2018)

The nucleus (cyan) as a mechanical gauge to probe pore sizes and thus enable migration along the path of least resistance. (GM-CSF-differentiated and LPS-stimulated dendritic cell (DC); PDMS-based microchannels attached to a non-coated glass surface;  the cellular nucleus is stained with Hoechst and shown in cyan; imaged with a Nikon Ecplise Ti.). 

(for further details, see Renkawitz et al, Nature 2019)